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Measurement of Calcium and Movement in Heart Cells

 Last Update: 2021-03-01
 Source: Internet
 Author: User

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calcium effect heart

ca h2po4 2

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Preparations of calcium-tolerant cardiac myocytes for studies on intracellular calcium ([Ca
2+
]
i
) signaling and contraction have been difficult owing to the susceptibility of these cells to the enzymatic digestion process. This often leads to the cells acquiring a bricklike contracted shape with a granular cytoplasm and membrane blebbing. A successful preparation results in single myocytes that do not show spontaneous contractions and that remain relaxed in Ca
2+
-containing buffers, indicating that the sarcolemmal membrane was not damaged during enzymatic digestion and led to membrane depolarization.

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