Metformin combined oxygen photodynamics make triple-negative breast cancer cells more likely to "see the light die"

Tri-negative breast cancer refers to breast cancer in which the results of the chemical examination of the immune tissue of the cancerous tissue are estrogen-like (ER), progesterone-like (PR) and the cancer gene Her-2.
breast cancer accounts for 10.0% to 20.8% of all breast cancer pathological types, has special biological behavior and clinical pathological characteristics, and has a worse prognosis than other types.
photodynamic therapy is a new method for treating breast cancer, by combining photosensitivity agents with the corresponding light sources to produce reactive oxygen components (reactive oxygen speciess, ROS), selectively destroying diseased tissue to allow cancer cells to "see the light dead", but also lead to the formation of a hypoxia state of tumor tissue, reducing the effectiveness of treatment.
Recently, the team of Professor Jin Hongjun of the Molecular Imaging Center of the Fifth Hospital affiliated with Sun Yat-sen University, in cooperation with the Cancer Center of the Fifth Hospital affiliated with Sun Yat-sen University (Common Communication Lu Weize, first author Yan Xiaofeng) explored a new method to improve the hypoxia problem in photodynamic treatment, and initially found that oxydygen combined metformin has therapeutic potential for triple-negative breast cancer.
the study was published in the Journal of Translational Medicine, Annals of Translational Medicine (ATM, IF:3.297).
paper entitled "Metformin and oxyphotodynamic as novel treatment approach for triple-negative breast cancer" (metformin combined oxygen photodynamics provide a new treatment for triple-negative breast cancer) the hypoxia state is produced during photodynamic therapy and lacks effective treatment methods.
of research is now focused on the improvement of light sources and the development of third-generation nanomaterials.
This study solves the problem of photodynamic hypoxia from the direction of photodynamic treatment equipment, delivers the resulting pure oxygen directly through physical oxygen production technology to specially designed gas, light and electro-integrated irradiators, and the synchronization of oxygen and irradiated light synergy in the treatment area, so that the local area maintains a stable oxygen-rich state, while avoiding the toxic side effects of nanomaterials.
study first explores the advantages of oxygen photodynamic therapy over photodynamic therapy in triple negative breast cancer.
MDA-MB-231 cells of triple-negative breast cancer cells were treated with amino acetylpropy (ALA)-mediated photodynamic (PDT) and oxygen photodynamic (OPDT) to calculate their 50% inhibition concentration (IC50) values of 10.62±1.22 mM and 7.71±1.26 mM, respectively.
compared with ordinary photodynamic therapy, oxygen photodynamic therapy inhibits the growth of cancer cells has obvious advantages.
inhibition of MDA-MB-231 cells by different concentrations of ALA (A), ALA-PDT(B) and ALA-OPDT(C), and (D) cell survival in the three groups above at different concentrations of ALA, compared with the control group (0 mM).
, P.lt;0.05;
(Photo Source: Reference 1) At the same time, Professor Jin Hongjun's team also found that metformin and a 5-aminoacetylaric acid (ALA)-mediated oxygen photodynamic therapy have synergies in the treatment of tumors, using the combined use of the two at different concentrations, which can be calculated by the CompuSyn software, the combined drug use index (CI) and lt;1.
confocus microscope can be seen under the oxygen photodynamic treatment group of the original PpIX expression increased, confirming the strengthening of the therapeutic effect, with the oxygen photodynamic therapy, oxygen supply can increase the oxygen free base or single-line oxygen production, resulting in the death of tumor cells.
fluorescence concentration microscope suggests that the therapeutic effect is most pronounced when metformin is used in association with ALA and oxygen photodynamics.
(A, D, G, J) primary pyrethrin IX (PpIX) ;(B, E, H, K) mitochondrial green fluorescent protein (MTG) ;(C, F, I, L) primary nipple IX (PpIX) plus mitochondrial green fluorescent protein (MTG).
PpIX has a wavelength of 610 ± 20 nm and an MTG emission wavelength of 530±30 nm.
(PhotoSource:Supplied) Therefore, the researchers believe that oxygen photodynamic therapy can alleviate the problem of hypoxia in photodynamic therapy by physically giving oxygen.
is a potential new treatment for triple-negative breast cancer, but the specific mechanisms need further study.
this study provides a preliminary basis for further exploration of the role of metformin combined oxygen photodynamic therapy in the treatment of relapsed or metastasis triple negative breast cancer.
: . . . Pei X, Wang X, Xian J, Mi J, Gao J, Li X, et al. Metformin and oxyphotodynamic therapy as a novel treatment approach for triple-negative breast cancer. Annals of Translational Medicine. 2020;8:1138-.