Methods for building mouse models of tumor immunotherapy.

On October 2, Li Peng of the Guangzhou Institute of Biomedicine and Health of the Chinese Academy of Sciences published the new animal model research results online in the international journal mAbs" "Establishment of the peripheral humanized humanized lung cancer models for studying efficacy of PD-L1/PD-1 targetedthed eithrapy".
the study explored ways to quickly build mouse models using peripheral blood lymphocytes, and for the first time established mouse models of the immune system and tumor tissue derived from the same patient.
in recent years, immunotherapy has become the latest direction for the treatment of tumors with the elucidation of the immunocheckpoint mechanism and the development of chimeric antigen receptor T-cell technology.
mouse model is a common model for researching and evaluating tumor immunotherapy, in which rebuilding the human immune system in mice is the key to the establishment of animal models.
However, the mouse model, which is widely used at present, does not have a uniform method and standard for rebuilding the human immune system, which hinders the preclinical research and efficacy evaluation of tumor immunotherapy.
in the study, the researchers first successfully rebuilt the immune system in mice with immunodeficiency using different sources of immune cells, and then further developed a heterotransplant model that carried tumors in lung cancer patients, and used antibodies targeted at PD-1/PD-L1 to evaluate the efficiency and therapeutic effectiveness of immune reconstruction.
the study is the first to compare differences in the efficiency of different methods in rebuilding the immune system and explore ways to quickly build mouse models using peripheral blood lymphocytes.
the mouse model, which is widely used at present, uses the cells of healthy donors to rebuild the immune system, produces immune rejection among tumor tissue, and does not accurately respond to the therapeutic effect of immunotherapy on tumors.
the first study to isolate invasive T cells from tumor tissue in lung cancer patients and co-transplant with tumors, creating an "upgraded" mouse model: carrying the same patient's immune cells and tumor tissue in the same mouse.
the research system compared the method of establishing mouse model of tumor immunotherapy, explored the advantages of using peripheral blood lymphocytes to quickly establish mouse model, and established a mouse model of the immune system and tumor tissue derived from the same patient, the new model can more realistically simulate the patient's internal environment, and provide a more accurate research and evaluation tool for tumor immunotherapy.
the research was supported by the Chinese Academy of Sciences' Strategic Pilot Project, Guangdong Province and Guangzhou City.
Source: Guangzhou Institute of Biomedicine and Health.