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Heterodera avenae Woll., cereal cyst nematode, CCN) is widely distributed around the world and is an important pathogenic nematode that is a serious hazard to wheat, barley and other pyrethroid crops and pastures.
ccN resistance-related genes are fewin in the existing wheat varieties, but they are abundant in its near-species.
-variable goat grass No.1 is a genus of the oather-based goat, which has good resistance to root-knot nematodes (RKN) and granule spore nematone (CCN) and is an important genetic resource in wheat resistance breeding research.
, Dr. Huang Qiulan, Ph.D., Longhai Research Group of Chengdu Institute of Biological Research, Chinese Academy of Sciences, under the guidance of researcher Yu Weiqun and assistant researcher Zhang Haili, took A reede.ilis No.1 as a material to perform functional analysis of the candidate gene AeVTDCs selected by THE RNA-Seq analysis screening induced by the early CCN infection.
TDC catalyzes tryptophan into trythamine, a precursor substance that synthesizes IAA and pyridine alkaloids, and is a key enzyme that connects plant primary metabolism with secondary metabolism.
the detection of the plant's anti-CCN levels and AeVTDC1 genetically modified tobacco anti-RKN capability through the VigS silence Ae.dililis No.1 plant root, and the results show that AeVTDC1 plays a positive regulatory role in CCN and RKN resistance reactions. Further analysis
found that there was no significant change in IAA biosynthetic genes, signal ingenuity and IAA content in silent plants and overexpression plants, and significant changes in the content of tDC downstream related metabolites and their synthetic gene expression levels.
speculate that the resistance regulation of the AeVTDC1 gene to CCN may be related to metabolites downstream of TDC, but not to IAA.
these results lay the foundation for revealing the molecular mechanism of anti-CCN, and also provide dispensing with the AeVTDC1 gene in molecular breeding of cereal crops.
the results of the study, published in the journal Frontiers in The Rbyer Agains Rinseilis No.1 Regulate the Resistance Against Cereal Cyst Nematode by The Terptophan decarnos
the research results were funded by the NSFC project, the GM project and the Western Ph.D. project.
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