N-Glycosylation of Plant Recombinant Pharmaceuticals
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Last Update: 2021-03-12
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Source: Internet
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Author: User
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N
-glycosylation is a maturation event necessary for the correct function, efficiency, and stability of a high number of biopharmaceuticals. This chapter presented here proposes various methods to determine whether, how, and where a plant pharmaceutical is
N
-glycosylated. These methods rely on blot detection with glycan-specific probes, specific deglycosylation of glycoproteins followed by mass spectrometry,
N
-glycan profile analysis, and glycopeptide identification by LC-MS.
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