Nankai University's new study will make pufferfish poisoning people "can save"

On May 28, Nankai University's National Key Laboratory for Drug Chemistry Biology led a research team, using monkey monoploid neural stem cells to successfully "crack" the two target genes of puffertoxin, and through gene knockout tests, nerve-like cells obtained puffertoxin resistance, this research will make the pufferfish poisoning "drug-saving", the results of which are published online in the latest issue of the academic journal Development in the field of stem cell and neurodevelopment.
the delicious, but the pufferfish toxin is extremely toxic, often causing the wrong eater to die instantaneously, its target and toxicology mechanism has so far been unknown.
existing studies suggest that puffertoxin is a kind of neurotoxin, its toxicology may be to block the sodium potassium ion channels in nerve cells and thus play a toxic role.
, can monkey hapos neurostem cells be used to screen for the "point of action" of puffertoxins on the nervous system? Led the team to reverse the trend, with the help of the emerging haploid cell tool culture technology in recent years, through the adoption of the initial state pluripotent culture system, so that the monkey haploid embryonic stem cells have better monoploid maintenance and in vitro differentiation process of cell survival, and in the world for the first time with in vitro proliferation capacity of the monkey monoploid neural stem cells.
experiment, the researchers then conducted a high-volume, whole genome random mutation of the monkey monoploid neural stem cells obtained, and conducted a puffertoxin poison-killing experiment on the mutant cell line.
finally, bioinformatics analysis and gene-editing experiments confirmed that the key target base for puffertoxins is B4GALT6 and SCN5A.
from a genetic point of view, because directly found the specific exercise of toxic function of functional genes, theoretically as long as these target genes knock edited off, nerve cells can obtain the resistance of puffertoxin.
reported that because of monkeys and other primates and human genetic similarity is very high.
For humans, finding a target gene can detoxify by taking gene inhibitors.
this also means that in the near future, people who have been poisoned by pufferfish will no longer be without medicine.
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