Necessary equipment for animal cell culture laboratory

Cytology, the necessary instrument of animal cell culture laboratory, plays an important role in life science In recent years, cytology has developed vigorously and made great progress The construction of cell laboratory is also the necessary laboratory of biology in various colleges and universities Cell experiments are cautious and complex, and there are many kinds of experimental equipment needed The following is a brief introduction of some necessary instruments in animal cell laboratory: * CO2 incubator is a necessary instrument for cell culture, which provides a comfortable environment for cell culture in vitro, such as suitable temperature, humidity, pH, O2 concentration, etc Cells are easy to be infected by various bacteria and microorganisms when cultured in vitro Therefore, the sterilization and antibacterial ability of CO2 incubator is particularly important Thermo Scientific 8000 Series CO2 incubator has professional sterilization and antibacterial functions Such as 140 ℃, 12hour dry heat sterilization, which can effectively kill all kinds of microorganisms; HEPA filter, which can achieve class 100 air quality in the cavity; stainless steel polishing inner tank, which can effectively inhibit the adhesion of microorganisms; silver ion disinfection box to prevent water in the water pan from contaminating bacteria, etc., which will escort your cell culture 2、 Double distilled water (more than 0.8m Ω) is generally required for cell culture in pure water system In fact, the double distilled water obtained by the second distillation of common distilled water in glassware according to the standard method can not meet the requirements Moreover, endotoxin in water directly participates in cell apoptosis The lower the content of endotoxin in serum, the less toxic it is to cells, and the better it is for cell growth and passage; If the endotoxin content is too high, it will directly lead to cell aging in advance Therefore, a formal water purification system is recommended Thermo Scientific Barnstead pure water machine has stable water quality, long service life of consumables and low transportation cost It is the choice of pure water system in your laboratory 3、 Cell culture of super clean worktable / biosafety cabinet has high requirements for sterile environment In cell operation, * generally needs to be carried out under 100 level air quality conditions, so super clean worktable or biosafety cabinet must be equipped ESCO super clean workbench and biosafety cabinet in Singapore provide you with more secure protection 4、 In order to preserve cells, especially mutant cells or cell lines that are not easy to obtain, liquid nitrogen tanks need to freeze the cells The temperature of cryopreservation is generally liquid nitrogen temperature - 196 ℃, so liquid nitrogen tank is needed In this environment, * like cells can be preserved for ten years and have activity 5、 The cryopreservation process of cryorefrigerator cells needs a series of procedures to cool down Normally, they are stored at 4 ℃ for 30 minutes, transferred to - 20 ℃ for 1 hour, then transferred to - 70 to - 80 ℃ overnight, and then transferred to liquid nitrogen (- 196 ℃), at this time, they must be equipped with * sets of - 86 ℃ cryorefrigerators *Cells can be preserved for several months in an ultra-low temperature refrigerator Cells that do not need long-term cryopreservation can be temporarily stored in an ultra-low temperature refrigerator Thermo Scientific Revco ultra-low temperature refrigerator has the refrigeration capacity of two 1HP industrial grade compressors; dual fan design has fast heat dissipation capacity, the environment can withstand 32 ℃ high temperature without alarm; key lock hole switch ensures the safety and reliability of the sample; as well as the humanized design of inner door, door lock, etc Revco ultra-low temperature refrigerator has a history of 70 years to create a professional and quality, worthy of the world * brand 6、 Constant temperature water bath is also a necessary instrument for cell laboratory 7、 Centrifuge cell culture needs centrifugation to collect subcultured cells, so a low-speed normal temperature centrifuge is needed, and a high-speed frozen centrifuge is needed for the separation of cell contents in the later stage 8、 In the experiments of pipette and cell culture, there are a lot of frequent pipette operations, so it is necessary to use all kinds of micro pipette, bottle mouth distributor and pipette controller All kinds of cell culture plates, cell culture bottles, pipettes and other consumables are even more indispensable, and * times, a large amount of consumption Brand is the world's manufacturer of pipette products and consumables, an expert in volume measurement in Europe, and Germany's brand pipette is one of the three major brands in the current pipette market * In addition to the above instruments, autoclaves and inverted microscopes are also necessary instruments for cell culture, and fermentation tanks and rotating bottles are also often found in cell culture laboratories Zhejiang Fuxia Medical Technology Co., Ltd., located in Daoxu Town, Shangyu District, Shaoxing City, Zhejiang Province, is a manufacturer in the purification equipment industry in East China According to iso14644-1 standard, GB50073-2001 national standard and GMP standard, we can provide professional air purification system engineering design, construction, testing and technical services for microelectronics, biomedicine, hospital operating room, optical fiber cable, food and beverage, precision instrument, semiconductor and new material application industries The company's main products are purification bench series, wind shower series, ventilation cabinet series, biosafety cabinet series, dust particle counter, planktonic bacteria sampler, which are widely used in medical and health, electronics, pharmacy, biology, food, agriculture and forestry, animal husbandry and veterinary, inspection and quarantine, aerospace, automobile manufacturing, precision instruments, universities and scientific research institutions