-
Categories
-
Pharmaceutical Intermediates
-
Active Pharmaceutical Ingredients
-
Food Additives
- Industrial Coatings
- Agrochemicals
- Dyes and Pigments
- Surfactant
- Flavors and Fragrances
- Chemical Reagents
- Catalyst and Auxiliary
- Natural Products
- Inorganic Chemistry
-
Organic Chemistry
-
Biochemical Engineering
- Analytical Chemistry
- Cosmetic Ingredient
-
Pharmaceutical Intermediates
Promotion
ECHEMI Mall
Wholesale
Weekly Price
Exhibition
News
-
Trade Service
Version 2.0 STOPCovid builds on the original test and further improves stopCovid.V2 detection sensitivity by adding RNA from the magnetic bead-rich sample during sample preparation.
in the initial STOPCovid testing process, Zhang Feng's team combined the iso-temperature PCR step of amplification of RNA numbers with a chemical reaction using Casase to detect specific sequences of the new coronavirus in the same test tube.
this test step does not require a complex instrument, only a constant temperature reaction of 60 degrees Celsius, you can read the test results through the side-flow test paper.
, however, in further tests, the researchers found that only 60.5 percent of patients tested positive for traditional RT-PCR were found in this simple version of the process.
To improve the sensitivity of the test, STOPCovid.v2 added steps to the process of collecting new coronavirus RNA through magnetic bead-rich nasopharyngeal swabs, using magnetic beads used in ordinary laboratories to collect all viral RNA contained in nasopharyngeal swabs as a starting material for reactions, and the researchers streamlined the process of collecting RNA from the magnetic beads, removing ethanol cleaning and wasting the entire RNA-rich process for no more than 15 minutes.
a blind test conducted at the University of Washington, researchers tested 202 new coronavirus-positive and 200 samples of new coronavirus-negative nasopharyngeal swabs in a blind test conducted at the University of Washington.
found that STOPCovid.V2 was 93.1 percent sensitive and 98.5 percent specific.
positive sample takes only 15-45 minutes to get results.
stopCovid.V2 test results, B: new coronavirus-positive sample; C: new coronavirus-negative sample, red dotted line to determine whether the sample is positive or negative threshold (Photo source: Resources 1)) Researchers said in an article that this simplified version of stopCovid.V2 process is suitable for clinical laboratories without complex instruments.
, the researchers also developed a smartphone APP that helps testers interpret the results of side-streaming paper.
resources: s1. Joung et al, (2020). Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing. NEJM, DOI: 10.1056/NEJMc2026172.