New protein interaction imaging technology: BiFC technology

Recently, Professor Chen Kuangshi's research group from the Department of Biomedical Engineering of the Future Technology College has developed a new imaging technology that can study protein interactions in cells at the nanometer scale based on the Bimolecular Fluorescence Complementation (BiFC) technology

BiFC technology is a method for imaging protein interactions in cells.

Figure 1.

"Through the development of a new super-resolution BiFC technology, we have discovered that the host cell AGO2 protein is involved in the whole process of HIV-1 virus particle assembly and is an indispensable element for virus proliferation

During the proliferation of HIV-1 virus, its structural protein Gag needs to interact with many host cell proteins to complete the assembly and budding of virus particles

Figure 2.

Mao Shiqi, Ying Yachen, and Ma Zhao of Chen Kuangshi's research group are the co-first authors of this work.