Rat nitric oxide synthase (T-NOS) enzyme-linked immunoassay (ELISA)

" reagents for research purposes onlyPurpose: This kit is used to determine rats serum, plasma and related liquid samples of nitric oxide synthetic enzymesT -NOS)contentcontent.

experimental principle:

kit is used with a doubleantibody clamping method to determine the rat nitric oxide synthase< "> <"">- <"">NOS""level. With purified rat nitric oxide synthase" (T - NOS"<antibody package ismicroplate, made into solid-phase antibodies, to the packet of monoantigen micro-holes in turn added nitric oxide Synthesis enzymes" <" (">- NOS")", andHRPmarked nitric oxide enzyme">" (T - NOS""antibody binding, forming antibodies- <" A href> ""- ase-labeled antibody complex, after thorough washing of the substrateTMBcolor. TMBis transformed into blue by the catalysis ofHRPenzyme, and into the final yellow with the effect of acid. The shade of color is positively related to the nitric oxide synthase">" (Tstyle"" >- Measure the absorbent at 450nm wavelengths" ( OD values"), The concentration of nitric oxide synthase <"">" ("T - NOS) was calculated by the standard curve.

" kit :

kit composition

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96