Rat nitric oxide synthase (T-NOS) enzyme-linked immunoassay (ELISA)

rat nitric oxide synthase (T -NOS enzyme-linked immunoanalytogenic analysis ( ELISA < "FONT-SIZE: 14.0pt;COLOR: black;">)

< "FONT-SIZE: 14.0pt;" > thebox instruction manual

reagents for research purposes onlyPurpose: This kit is used to determine rats serum, Nitrogen monoxide synthase(T -NOS) in plasma and related liquid samplesthecontent.

experimental principle:

> kit is used in a doubleantibody sandwiching method to determine the rat nitric oxide synthase(T - <"">NOS"Level. With purified rat nitric oxide synthase" (T - NOS"<antibody package ismicroplate, made into solid-phase antibodies, to the packet of monoantigen micro-holes in turn added nitric oxide Synthesis enzymes " <" (">- NOS") ", and HRP "labeled nitric oxide ">" (T - NOS"" antibody binding, forming antibodies - <" A href> "" - ase-labeled antibody complex, after thorough washing of the substrate TMB color. TMB is transformed into blue by the catalysis of HRP enzyme, and into the final yellow with the effect of acid. The shade of color is positively related to the nitric oxide synthase ">" (T style"" >- . Measure the absorbent at <"">450nm wavelengths" ( OD value"), Using the standard curve, the concentration of nitric oxide synthase <-