Oil red O lipid staining method.

oil red O belongs to acetic nitrogen dye, is a strong lipid solvent and grease dye, and triglyceride binding to a small fat drop. Fat-soluble dyes are soluble in
tissues
and cells, and are more soluble in lipids than in solvents. When the tissue
slices
a human dye, the dye leaves the dye and dissolves in the lipids (such as fat droplets) in the tissue, making the fat droplets in the tissue orange., the preparation of oily red O dye
1. Oil red O1g
isopropyl alcohol 100ml
2. Oil red O sufficient amount
isopropanol 100ml
is assigned to oil red O saturation solution, when used with oil red O saturated liquid one plus two servings of distilled water,
filtration
after use.2nd, dyeing procedure
1. Slices are fixed with formaldehyde-calcium for 10 minutes;
2. Distilled water wash;
3. 60% isopropyl alcohol immersion;
4. 1 by red O dye 10 minutes (dye can be recycled);
5. 60% isopropyl alcohol is colored to the background five colors;
6. Distilled water wash;
7. Mayer Sumu fine re-dyeing;
8. Tap water wash (blue) 1 to 3 minutes;
9. Distilled water wash;
10. Glycelue gelatin seal.
result: the fat droplets in tissue cells are orange and the cores are blue.oil red O lipid staining
shows
rats
fatty liver in the liver cells infected with red .