On October 12, 2021, Fang Dong’s laboratory clarified in Nature Communications that H3K27me3 as a key regulatory factor plays an important role in the asymmetric cell division process induced by Wnt3a

The Wnt signaling pathway plays a very important role in the growth and development of stem cells, maintenance of stemness, tumorigenesis and metastasis

2021 Nian 10 Yue 12 days, I was in the hospital laboratory Eastern Nature Communications , published online entitled "Joint single-cell multiomic analysis in Wnt3a induced asymmetric stem cell division" of research papers

SET-seq first isolation of nuclei of the same cell and cytoplasmic RNA , then based on Tn5 transposase, the nucleus of the transfected protein bound chromatin fragments be apparent library construction ; while inverting synthesized cytoplasmic RNA of the cDNA , using Tn5 transfer The enzyme constructs a transcriptome library on the cDNA/RNA hybrid strand (Figure 1A )

Researcher Fang Dong is the corresponding author of this article.

FIG 1 through S the ET - SEQ confirmed that in the Wnt .

Original link:

https://doi.

references:

1Habib, SJet al.

2Ma, B.