One hour results! Zhang Feng team released CRISPR detection technology of new coronavirus

Author: the novel coronavirus (COVID-19) outbreak of Gu Lulu has brought great challenges to global health In support of the war, "epidemic", on February 14, the broad Institute of Massachusetts Institute of technology, together with Harvard University, the McGovern Institute of Massachusetts Institute of technology and its related partners announced that they would provide information that might be useful free of charge, including through sharing information that might support the development of potential diagnostic technologies Among them, there is a detailed operation flow document of CRISPR based diagnostic covid-19 technology developed by Professor Zhang Feng, gene editor of CRISPR, Omar abudayeh and Jonathan gootenberg At present, the novel coronavirus can be diagnosed by qPCR However, the detection of diseases is slow because of lack of reagents and equipment CRISPR based covid-19 diagnostic technology uses Sherlock technology released by Professor Zhang Feng's team in 2017 Previously, Sherlock system based on crispr-cas13 has been proved to be able to accurately detect a large number of different viruses in patient samples The system searches for unique nucleic acid features and uses a test paper similar to a pregnancy test paper to provide a visual reading After immersing the test paper in the prepared sample, a line will appear on the test paper to indicate whether there is virus Based on this theory, the team designed and tested two kinds of guide RNAs to recognize the two characteristics of covid-19 using the synthesized covid-19 RNA fragment One recognizes the S gene of the new coronavirus, the other recognizes the orf1ab gene When they bind to cas13, they form a Sherlock system that can detect covid-19 RNA It is easy to confirm the existence of the new coronavirus through the different positions of the bands The system has specific high sensitivity, only 10-100 virus copies per microliter can be detected And the steps are simple, only three steps, within an hour can complete the detection: · using the conventional 42 ℃ homothermal amplification technology, the extracted nucleic acid samples can be amplified This step will take 25 minutes ·The sample in step 1 was added with cas13 protein, guide RNA and special molecules for reporting, and the reaction continued for 30 minutes at 37 ℃ ·Use the test paper to test the sample obtained in step 2, and the result should appear within 5 minutes It is worth noting that this technique has not been validated on patient samples Any diagnosis needs to be developed and validated for clinical use Professor Zhang Feng's team welcomes researchers to work with them and can provide a starter kit to test the system If possible, it can also be used by researchers testing covid-19 samples Full text of technical documents attached: Reference: [1] enabling coronavirus detection using crispr-cas13: an open access Sherlock research protocol [2] a protocol for detection of virus-19 using CRISPR diagnostics [3] what are the three steps of new coronavirus CRISPR detection technology of Zhang Feng's team? Jeni turtle