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One-Step Purification and Biochemical Characterization of Recombinant Pancreatic Lipases Expressed in Insect Cells

 Last Update: 2021-01-05
 Source: Internet
 Author: User

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The baculovirus expression system is very convenient to produce recombinant pancreatic lipases and mutants thereof, in substantial amounts (10–50 mg of enzyme per liter of culture) for structure-function studies (
see
also Chapters 17 , 18 , and 20 in this volume). Using the naturally occurring leader sequence, recombinant enzymes are secreted by insect cells and are easy to purify in a one-step procedure when insect cells are grown in a serum-free medium.

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