Operation guide and precautions of the exhaust type anaerobic tank

HP series anaerobic tank is a suction type tank body, which can be used as a suction tank or as a closed tank If HP series anaerobic tank is used as a closed tank (please pay attention to the quick air extraction connector and connecting pipe configured with the tank, and protect the quick plug-in connector on the tank cover to avoid contamination): 1 Please place the sample plate to be cultured on the bracket and put it into the tank, or put other samples to be cultured such as test tube, small triangle bottle, etc into the tank According to the operation instructions, tear off the outer package and put the inner bag into the tank, quickly cover the tank cover and slide the clamp fixing the tank cover to clamp the tank cover, and tighten the clamp knob clockwise by hand Please note that the positioning rod under the fixture should correspond to the positioning groove on the tank cover When tightening, it can rotate down slightly after feeling resistance, without excessive force 2 Put the anaerobic tank into the incubator with set temperature, and the culture time depends on the growth characteristics of the bacteria to be cultured 3 At the end of cultivation, take the sundries such as production air bag, wash the tank with water, dry and reserve 　　 If it is used as an air extracting tank (use the quick connector and connecting pipe equipped with the tank and check the air outlet pressure of the pressure reducing valve of the gas cylinder 0.05mp): when inserting and pulling out the quick connector, please note: when inserting, please directly press the connector with the connecting pipe under pressure and insert it into the quick connector base on the tank cover, do not screw it in When disconnecting, please hold the connector with one hand First, press down the outer wall of the quick connecting base on the tank cover with the other hand to eject the snap in head from the base, do not rotate and twist it! 1 Place the sample plate to be cultured on the support and put it into the tank, or put other samples to be cultured, such as test tubes, etc., into the tank Please pay attention to the air permeability of disposable flat dish 2 If it is necessary to observe the oxygen concentration in the tank, the corresponding indicator can be put into the tank according to its operation instructions 　　 3 Close the tank cover tightly and slide the clamp fixing the tank cover to clamp the tank cover, tighten the clamp knob clockwise, connect the culture tank and the vacuum pump through the quick air extraction pipe, start the vacuum pump according to the operation instructions of the vacuum pump (it is recommended to use the HT special anaerobic tank suction pump, Zui large vacuum degree is less than 0.1bar or use the labjar automatic suction device), and pump the tank to the negative pressure of about 0 6-0.8 bar, about 40-45 seconds, then pull off the quick connect connector of the tank cover, and connect the mixed gas or user-defined gas through the quick gas connection pipe after reducing the pressure of the secondary gas pressure reducing valve (pay attention to adjust the outlet pressure to 0.05mp) The charging time is generally less than 5 seconds Pay attention to the pressure gauge display of about 0.02mp, pay attention to the charging pressure and charging time Do not exceed the value, otherwise it is easy to cause tank damage Close the air supply and disconnect the gas connector of the tank cover immediately after inflation 4 Put the anaerobic tank into the incubator with the set temperature, and the culture time depends on the growth characteristics of the bacteria to be cultured 5 At the end of culture, take out the culture dish, wash the tank with water, dry it and reserve it Note: the pressure gauge provided with the tank is a simple type At the beginning, the pointer is mostly not at 0 position, and the error within ± 0.02MPa does not affect the use Note: 1 Alcohol is forbidden to wipe the incubator 2 The high temperature resistance of the culture tank Zui is generally no more than 80 ℃; it is forbidden to touch the tank with sharp and sharp things 3 Please clean the sealing ring in the tank cover groove according to the use situation and apply a thin layer of acid free Vaseline or silicone grease evenly with fingers or swabs 4 It is recommended to use mild and neutral cleaning disinfectant or other suitable products when cleaning and disinfecting the tank body After cleaning and disinfecting the tank body, use clean water to wipe or wash it 5 Do not let the tank fall on the hard surface, check the tank regularly for cracks or damages, check the sealing ring regularly for dirt, and update it in time.