Operation steps of autoclave

Operation step 1 First take out the inner sterilization barrel, and then add some water into the outer pot to make the water surface level with the triangular shelf 2 Put back the sterilization barrel and put the articles to be sterilized Pay attention not to pack too crowded, so as not to hinder the flow of steam and affect the sterilization effect The triangle flask and the mouth end of the test tube shall not contact with the barrel wall, so as to prevent the condensed water from wetting the paper at the mouth of the package and penetrating into the cotton plug 3 Cover and insert the exhaust hose on the cover into the exhaust slot of the inner sterilization barrel Then tighten the two opposite bolts at the same time in a symmetrical way, so that the bolts are tightened in the same way without air leakage 4 Use electric stove or gas to heat, and open the exhaust valve at the same time to make the water boil to remove the cold air in the pot When the cold air is completely exhausted, close the exhaust valve, so that the temperature in the pot increases gradually with the increase of steam pressure When the pressure in the pot rises to the required pressure, control the heat source and maintain the pressure for the required time In this experiment, 1.05kg/cm2121.3 ℃ was used for sterilization for 20 minutes 5 When the time for sterilization is up, cut off the power supply or turn off the gas to make the temperature in the sterilization pot drop naturally When the pressure of the pressure gauge drops to 0, open the exhaust valve, loosen the bolt, open the cover and take out the sterilization articles If the pressure does not drop to 0, open the vent valve, the pressure in the pot will suddenly drop, so that the culture medium in the container will rush out of the flask mouth or tube mouth due to the imbalance of the internal and external pressure, causing the cotton plug to contaminate the culture medium 6 Put the sterilized medium out into a 37 ℃ incubator for 24 hours If there is no growth of mixed bacteria, it can be used First, take out the inner sterilization barrel, then add some water into the outer pot to make the water surface level with the tripod 2 Put back the sterilization barrel and put the articles to be sterilized Be careful not to pack too tightly, so as not to hinder the steam flow and affect the sterilization effect The triangle flask and the mouth end of the test tube shall not contact with the barrel wall, so as to prevent the condensed water from wetting the paper at the mouth of the package and penetrating into the cotton plug 3 Cover and insert the exhaust hose on the cover into the exhaust slot of the inner sterilization barrel Then tighten the two opposite bolts at the same time in a symmetrical way, so that the bolts are tightened in the same way without air leakage 4 Use electric stove or gas to heat, and open the exhaust valve at the same time to make the water boil to remove the cold air in the pot When the cold air is completely exhausted, close the exhaust valve, so that the temperature in the pot increases gradually with the increase of steam pressure When the pressure in the pot rises to the required pressure, control the heat source and maintain the pressure for the required time In this experiment, 1.05kg/cm2121.3 ℃ was used for sterilization for 20 minutes 5 When the time for sterilization is up, cut off the power supply or turn off the gas to make the temperature in the sterilization pot drop naturally When the pressure of the pressure gauge drops to 0, open the exhaust valve, loosen the bolt, open the cover and take out the sterilization articles If the pressure does not drop to 0, open the vent valve, the pressure in the pot will suddenly drop, so that the culture medium in the container will rush out of the flask mouth or tube mouth due to the imbalance of the internal and external pressure, causing the cotton plug to contaminate the culture medium 6 Put the sterilized medium out into a 37 ℃ incubator for 24 hours If there is no growth of mixed bacteria, it can be used