Optimisation of liquid fermentation media for side spores cytobacteria

, experimental purposes 1, master the principle of fermentation medium preparation,2, familiar with the method of optimizing fermentation medium: orthogonal test, uniform design, response surface method, secondary orthogonal rotation methodThe main use of orthogonal test, but also can use other methods, the experimental principlethe fermentation medium refers to the medium used in large-scale production, because the fermentation product generally contains a higher proportion of carbon elements, so the carbon content in the medium should also be higher than the seed medium, if the product has a high nitrogen content, should also increase the proportion of nitrogen in the mediumHowever, it is necessary to pay attention to the osmotic pressure in the medium, if the osmotic pressure is too high, it will in turn inhibit the growth of microorganisms, in which case the flow plus method can be gradually added carbon nitrogen sourcethe media components play a critical role in fermentationIndustrial fermentation media and the use of bacteria screening of the medium is different, generally based on economic savings as the main principle, so often used as a cheap agricultural and sideline products as raw materialsWhen choosing carbon sources, potato flour, bran, cornflour, etcare commonly used instead of starch, and soybean meal, bean cake powder, etcas nitrogen sources, in addition, the selected raw materials should not affect the downstream separation extraction workDue to the complex composition of these natural raw materials, different batches of raw material composition are different, before fermentation must be carried out in the optimization test of the medium, experimental equipment and reagents 1, bacteria species: side spore cytobacteria sloping bacteria species;2, seed culture medium: sucrose 1.5%, protein 0.2%, yeast paste 0.4%, calcium carbonate 0.5%, hydrophosphate 0.1%, magnesium sulfate 0.03%, manganese sulfate 0.002%;Base: reference seed medium research;4, counting plate: beef paste protein medium;5, experimental equipment: balance, portable electric sterilization pot, ultra-clean workbench, inoculation ring, electric blowwind drying tank, temperature-controlled shaker, optical microscope, electric furnace, alcohol lamp, glass funnel, test tube, triangular bottle, glass 4, experimental steps (orthogonal test) 1, determine factors and levels: selection of sucrose, protein, yeast paste as the main factors ;: Regardless of interaction, random arrangement, 4, listed test scheme, 5, test: culture condition180r/min, 37 degrees C 80% of the bacteria to form buds, end culture, 6, orthogonal test results of statistical analysis Five, Notes 1, conditions need to carry out single-factor testing; 2, culture temperature, shaker speed, bacteria and inoculation volume and other results have a greater impact, the conditions need to be consistent