Orange peels and 5-hydroxy-6, 7, 8, 3', 4'-methoxyflavone are suppressed by activating the ROS-AKT/mTOR signaling path

Rheumatoid arthritis (RA) is a long-lasting and recurrent autoimmune disease. The School of Food Science and Engineering, Central South Forestry University,
Rice and By-products
Seed
Processing National Engineering
Laboratory Guliang Yang, Haiyan Zhong and Shiming Li of Huanggang Normal College, etc., based on the rat RA model induced by bovine type II collagen (BIIC) Tangeretin, TAN) and 5-hydroxy-6, 7, 8, 3', 4'-methoxyflavone (5-hydroxy-6, 7, 8, 3', 4' pentamethoxyflavone, 5-HPMF) have a preventive effect on RA and its molecular mechanisms. After 5 weeks of INTERVENTION by TAN or 5-HPMF (TAN/5-HPMF), RA lesions and autophagy levels in the membrane tissue were significantly reduced, while ROS content and HO-1 expression levels were decreased. After TAN/5-HPMF feeding, the relative expression levels of p-AKT and p-mTOR were lowered. At the same time, the relative expression level of p62 in the TAN/5-HPMF treatment group was more than 2 times higher than that of the BIIC group. Immunofluorescent staining and Western blotting results further confirm that TAN/5-HPMF treatment reduces BIIC-induced conversion from LC3I to LC3II. Observations under a transmission electron microscope also showed a decrease in autophagy levels under TAN/5-HPMF intervention. These results show that TAN and 5-HPMF prevent the pathological process of BIIC-induced arthritis by inhibiting autophagy of membrane cells achieved by the ROS-AKT/mTOR signal axis. Therefore, the study confirmed the protective effects of TAN and 5-HPMF on RA disease.introduction to
Communication Authors: Professor
Li Shiming
Li Shiming, Ph.D. in Food Science, Rutgers University, Texas Agricultural and Industrial University, Bachelor of Chemistry, Department of Chemistry, Nanke University, Tianjin; He has worked in the pharmaceutical research and development departments of Johnson and Johnson and Roche Pharmaceuticals in the United States, developing new drugs to fight cancer and treat metabolic syndromes such as diabetes and obesity, researching and developing functional foods such as health food, medical food and functional beverages for many years, and has published several papers, including SCI, as well as several patent applications and chapters. The main research areas are functional food and product development, natural product chemistry, plant compound separation, analysis, structural identification, drug metabolism and bioactive evaluation of compounds. Two U.S. patents have recently been granted: one for the treatment of lung cancer using a combination of pharmaceutical and food active ingredients, and the other for the treatment of diabetes with styrene glycosides.
Zhong Haixuan,
,
Zhong Haixuan,
a
-level professor and doctoral tutor. Domestic well-known food forest products processing and utilization experts. The subject leader of Hunan Province's general colleges and universities, the national science and technology award evaluation experts. Member of the Standing Committee of the China Forest Food Science and Technology Committee and member of the 9th Hunan Provincial Science and Technology Association. Senior Research Scholar, Food Ingredients and Methods Development Laboratory, USDA, Visiting Professor, Charles Sturt University, Australia, Postdoctoral Tu Graz, Austria.
academic achievements:
chaired the completion of the National "11th Five-Year Science and Technology Support" project 1 and the National Natural Science Foundation of China 3 projects. He has wrote 6 academic monographs at home and abroad, including
.
,
. Agric. Food Chem has
more than 170 papers published in major academic journals at home and abroad, such as the China Journal of Grain and Oil and the Journal of Crop Science. Won the provincial (ministry) one, two and three levels of science and technology awards 4. Through international cooperation in oil tea and research on projects such as the National Natural Science Foundation of China, important research progress has been made in the formation of tea oil quality and the by-product utilization of oil tea.
Article "Protective Effect of Tangerentin and 5-Hydroxy-6,7,8,3', 4'-Pentamethoxyflavone on Collagen-Induced Arthritis by Resing Autophagy via Activation of the ROS-AKT/mTOR Signaling Pathway is published online at
Journal of Agricultural and Food Chemistry
。