PCR and RFLP Methods for the Specific Detection and Identification of Mycoplasma mycoides subsp. mycoides SC
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Last Update: 2021-02-15
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Source: Internet
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Author: User
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The polymerase chain reaction (
PCR
),
DNA
hybridization, and sequence analysis have been valuable in the study of the phylogenic relationships between members of the
Mycoplasma mycoides
“cluster” (
1
). They have confirmed the very close relationships between these organisms suggested by others (
2
,
3
), and in the case of
M. mycoides
subsp.
mycoides
SC (
Mmm
SC), provided a rapid diagnostic test for the detection and identification of this organism based on DNA amplification by PCR and restriction fragment length polymorphism (RFLP) between
Mmm
SC and (
Mmm
LC) (
4
).
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