PEG precipitation method purification virus
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Last Update: 2021-01-21
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Source: Internet
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Author: User
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1, using ultrasound or freezing methods to break the cells to release the virus;
2, slowly stirring and adding NaCl final concentration is 0.5mol/L, helping the precipitation of the virus, and then the same amount added 10% PEG (generally used PEG6000 on it). If the purity of the sample is not strictly required, it can be recommended to use Millpore's
centrifugal enrichment
column, the Amicon series can concentrate 15 ml of samples at a time.
3, 4 degrees C overnight or placed for a period of time;
4, 8000/min centrifugation for 30 minutes to collect viral precipitation;
5, the virus precipitation into the appropriate amount of pbs, 4 degrees C overnight;
6, 10000r/min centrifugation 1 hour, precipitation is concentrated virus.
can also be further purified by gradient centrifugation or other methods.
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