Peptide Detection and Structure Determination in Live Cells Using Confocal Raman Microscopy
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Last Update: 2020-12-14
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Source: Internet
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Author: User
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Peptides are an important class of bioactive compounds that continue to be developed for a variety of therapeutic uses. Thebioactivity of peptides stems in most cases from their ability to enter or bind to the surface of cells to elicit a cellularresponse, and the primary sequence and secondary structure of the peptide determine this. Therefore, experimental methodsthat can provide structural information on peptides in live cells are useful for exploring peptide structure–activity relationshipsand metabolism directly within the targeted cellular environment. In this chapter we describe an experimental methodologyfor the detection and structure determination of exogenous peptides within living cells using confocal Raman microscopy (CRM). CRM is Raman spectroscopy performed under a confocal microscope. Raman spectroscopy itself has been applied to the study ofpeptides for several decades and provides a wealth of information, including secondary structure via the amide backbone vibrationalmodes, cysteine redox status via the S–S and S–H stretches, and disulfide conformation via the S–S stretch. The Raman spectraof peptides are dominated by intense bands associated with the aromatic ring vibrations of Phe, Tyr, and Trp. The positionsand intensities of some of these bands are sensitive to the hydrophobicity and pH of the peptide environment and thus canpotentially be used as intracellular probes. Heavy-isotope labeling of aromatic ring side chains shifts the spectral positionsof the aromatic ring vibrations and enables unambiguous detection of the peptide within cells. We employ this method primarilyfor the study of cell penetrating peptides in live cells. However, the method could in principle be applied to the study ofany type of peptide within any type of cell if the intracellular concentration of the peptide reaches high enough levels toenable detection.
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