Plant gene transport has a new method of magnetic nanoparticles as a carrier.

According to the latest news of the Chinese Academy of Agricultural Sciences, the Institute of Agricultural Environment and Sustainable Development of the Institute of Agricultural Environment and Sustainable Development and the scientific research team of the Institute of Biotechnology carried out joint research, using magnetic nanoparticles as a gene carrier, created a new method of high throughput, convenient operation and widely used plant genetic transformation, and promoted the research of nanocarrier gene transmission and genetic guidance system, and opened up a new direction of nano-biotechnology research.
research was published online November 27 in the authoritative academic journal Nature-Plant. According to the
, the development of efficient and safe new genetic transformation methods has been a hot topic in the fields of genetic engineering, molecular biology and genetic breeding.
traditional plant genetically modified methods, it is usually necessary to compare complex tissue culture and other plant regeneration procedures, in order to obtain genetically modified plants, especially cotton and other hard-to-regenerative crops of genetically modified plants preparation is more difficult.
the research and development of the magnetic nanoparticle gene carrier based on the pollen magnetic transformation plant genetic modification method, can use magnetic nanoparticle Fe3O4 as a carrier, under the guidance of the external magnetic field, the external gene is transmitted to the pollen inside, through artificial pollination using natural reproductive process directly to obtain the conversion seed, and then through the selection to obtain stable genetic ally genetically modified offspring.
this method combines nanomagnetic transformation with pollen mediating method, breaks through the bottleneck of traditional genetically modified method tissue regeneration culture and host adaptability, can improve the efficiency of genetic transformation, shorten the incubation cycle of genetically modified plants, realize high throughput and multi-gene coordination and transformation, the scope of application and use is very extensive, is of great significance for accelerating the cultivation of new varieties of genetically modified organisms, and has a wide range of applications in the fields of crop genetics, synthetic biology and bioreactors.
the research results have been funded by the national major special project son of genetically modified, the national 973 plan and the scientific and technological innovation project of the Chinese Academy of Agricultural Sciences.
Source: Science Daily.