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Plant phospholipases can be grouped into four major types, phospholipase D, phospholipase C, phospholipase A1 (PLA
1
), and phospholipase A2 (PLA
2
), that hydrolyze glycerophospholipids at different ester bonds. Within each type, there are different families or subfamilies of enzymes that can differ in substrate specificity, cofactor requirement, and/or reaction conditions. These differences provide insights into determining the cellular function of specific phospholipases in plants, and they can be explored for different industrial applications.