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Human procalcitonin (PCT) enzyme-linked immunoassay (ELISA)

 Last Update: 2020-11-22
 Source: Internet
 Author: User

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pre-calcitonin (PCT) ase-linked immunoanalytic analysis ( < FONT-SIZE: 14.0pt;COLOR:black;" > ELISA )

< ""> "reagents box instruction manual

< b style"""> reagents are for research purposes onlyPurpose: This kit is used to determine the content of humanserum, plasma and related liquid samplespre-calcitonin(PCT)content.

Experimental Principle:

kits are used in a doubleantibody sandwich method to determine the specimenpeople before calcitonin(PCT)level. Color with purifiedpeople before calcitonin < "color: black;" > (PCT)antibodypack ismicroplate , to make solid-phase antibodies, to the packet of monoantigen micropores in turn added pre-calcitonin (PCT) ">, and then HRP "">" labeled ex-calcitonin (PCT)" antibody binding, forming antibody - "" antigen - ase-labeled antibody complex, after thorough washing of the substrate TMB color display. TMB is transformed into blue by the catalysis of HRP enzyme, and into the final yellow with the effect of acid. The shade of color and the < "> before calcitonin < "color: black;" > (PCT) is positively related. Measure the absorbent at <">450nm wavelengths" ( OD < "" >" < value), using a standard curve to calculate the contents of the sample persons before calcitonin < "color: black;" > (PCT)

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kit composition

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