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 Home > Biochemistry News > Biotechnology News > Precise large deletions by the PCR-based overlap extension method

Precise large deletions by the PCR-based overlap extension method

 Last Update: 2021-02-28
 Source: Internet
 Author: User

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simple sop template

pcr extension time

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The authors describe an efficient method for generating large deletions (>200 nts) of precise length using the
PCR
-based method of gene splicing by overlap extension (
1
). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparation of a single-stranded
DNA
template.

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