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Preparation and Assay of Recombinant Serine Racemase

 Last Update: 2020-11-29
 Source: Internet
 Author: User

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serine

glutamate receptor

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Serine racemase is a glial and neuronal enzyme that reversibly converts
l-serine to
d-serine, an endogenous co-agonist of
N
-methyl-
d-aspartate receptor type glutamate receptors (NMDARs). Here we present methods to recombinantly express and purify serine racemase in bacteria and two complementary ways to determine
d-serine levels in unknown samples. Furthermore, a detailed protocol of serine racemase activity assays is described that can be used to screen for activators and inhibitors in vitro.

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