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Preparation of Chromatin Assembly Extracts from Xenopus Oocytes

 Last Update: 2021-02-22
 Source: Internet
 Author: User

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The majority of
DNA
in eukaryotic cells is packaged by histones and many poorly characterized nonhistone proteins to form a dynamic structure known as chromatin. Chromatin is a periodic structure made up of repeating, regularly spaced subunits, the nucleosomes. Elegant genetic experiments have clearly demonstrated that histones play a central role in transcriptional control (
1
). Moreover, histones, via protein-protein interactions or by playing an architectural role, can facilitate or inhibit the transcriptional activation process (
1
,
2
). It also appears that the function of histones themselves may be regulated by protein modifications and therefore may be targets for cell signaling pathways (
3
,
4
).

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