Preparation of glassware before sterilization

In the preparation of glassware before sterilization in the laboratory, there are requirements for the glassware used in the production of microbial culture medium For example, these glassware must be heat-resistant Of course, thorough sterilization must be carried out before use, so as to avoid affecting the experimental effect and the quality of the culture medium, causing unnecessary experimental impact There are mainly the following glassware used in the production of culture medium in the laboratory: culture dish, usually wrapped with kraft paper, 10 to 12 in a package, sterilized at 121 ℃ for 20 minutes, or 160 ℃ for two hours, dried for standby Tube, this is a common Zui, before sterilization, we need to add a cotton plug, size matters, and then sterilization Straw and capillaries In order to avoid infection of straw and capillaries in application, a little cotton is usually plugged at the mouth of the pipe Other glassware, for some glassware that is not easy to clean, can be soaked in some cleaning solution for a period of time, then placed in sodium hydroxide solution for a few minutes, neutralized in acid solution, washed with water several times after Zui and placed in the oven for drying Whether the glassware should be infected by bacteria? If it is infected, it should be sterilized by boiling sterilization and other sterilization methods before washing, and then cleaned