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phenol/chloroform solution
"Preparation Method"
mixing volumes such as phenol and chloroform with 0.1mol/L Tris? HCl (pH7.6) pumped several times to balance the mixture, placed in a brown glass bottle, covered with an equal volume of 0.01mol/l Tris? HCl (pH7.6) liquid layer, stored at 4 degrees C.
""note"
phenols are highly corrosive and can cause severe burns, and wear protective clothing > when operating withand protective glasses. All operations should be carried out in a chemical ventilation cabinet. Skin in contact with phenols is cleaned with plenty of water and washed with soap and water, avoiding ethanol. (Responsible Editor: King Kunlun of Great Han) |