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Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild-Type and Mutant Mitochondria: Activity Measurement and Subunit Composition Analysis

 Last Update: 2020-12-13
 Source: Internet
 Author: User

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saccharomyces cerevisiae characteristics

chain measurement

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The mitochondrial oxidative phosphorylation involves five multimeric complexes imbedded in the inner membrane: complex I (NicotinamideAdenine Dinucleotide (NADH) quinone oxidoreductase), II (succinate dehydrogenase), III (ubiquinol cytochrome
c
oxido reductase or
bc1
complex), IV (cytochrome
c
oxidase), and V (ATP synthase). These respiratory complexes are conserved from the yeast
Saccharomyces cerevisiae
to human with the exception of complex I, which is replaced by three NADH dehydrogenases in
S. cerevisiae
. Here, we provide several protocols allowing an exhaustive characterization of each yeast complex: this chapter describesprocedures from mitochondria preparation to measurement of the activity of each complex and analysis of their subunit compositionand provides information on the interactions between different complexes.

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