Preparation of slide strains for yeast detection.

Place a piece of circular filter paper on the bottom of the petri dish and place a U-shaped glass rod on top of it with two clean slides.
sterilization, a sterile way on the filter paper to add a few milliliters of sterilization of 20% oil solution, its main role is to maintain the wetting of the filter paper during the culture process.
a Pasteur pipe to add melted yeast-soaked agar or potato glucose agar to the surface of the slide to form a very thin agar culture base.
the yeast to be tested with an inoculation ring line is placed on the slide, cover part of the media with a sterile cover slide, and place the lid of the petri dish on a culture of more than 7d at 25 degrees C.
the growth status of the growing bacteria with a difference microscope, and compared the growth patterns of the bacteria exposed to the air and under the cover glass.
can also gently remove the cover glass from the slide, drop a drop of lactol, lactol-bitter acid or lactol cotton blue to fix the bacteria, and then cover the cover glass, the edge sealed with paraffin, nail polish or a special adhesive.
1, wipe the slide, cover slide.
2, drop a drop of water in the center of the slide.
3, pick a little mycelium with an anatomical needle.
4, coated in water droplets.
5, covered slides.
6, need to dye to be dyed, do not dye can also be observed.
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