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Tris buffers with various pH values
| 19.9 | .||||||||||||||||||||||||||||
8.4 | 17.2 | ||||||||||||||||||||||||||||
8.5 | 14.7 | ||||||||||||||||||||||||||||
8.6 | 12.4 | ||||||||||||||||||||||||||||
8.7 | .10.3 | ||||||||||||||||||||||||||||
8.8 | 8.5 | ||||||||||||||||||||||||||||
8.9 | 7.0 |
0.05mol/L Tris buffer preparation for a particular pH: 50ml 0.1mol/L. Tris alkali solution is mixed with 0.1 ml/L HCl of the corresponding volume (in ml) shown in the table above, and the added water brings the volume to 100m
temperature on 50mmol/L Tris-HCl liquid pH
25 degrees C
37 degrees C
.8. 1
7. 5
7. 2
8. 2
7. 6
7. 3
8. 3
7. 7
7. 4
8. 4
7. 8
7. 5
8. 5
7. 9
7. 6
8. 6
8. 0