 Home > Biochemistry News > Plant Extracts News > Preparing SC Spreads with RNs for EM Analysis

Preparing SC Spreads with RNs for EM Analysis

 Last Update: 2020-11-14
 Source: Internet
 Author: User

Tags

using

transmission electron

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

Recombination nodules (RNs) are associated with synaptonemal complexes (SCs) during early prophase I of meiosis. RNs are too small to be resolved by light microscopy and can be observed directly only by electron microscopy. The patterns of RNs on SCs can be analyzed using three-dimensional reconstructions of nuclei using serial thin sections, but this method is time consuming and technically difficult. In contrast, spreads of SCs are in one plane so all RNs in each set can be visualized simultaneously, and the patterns of both early and late nodules (ENs and LNs) can be analyzed far more easily than using sections. Here, we describe methods for preparing spreads of SCs and RNs from tomato primary microsporocytes on plastic-coated slides for visualization by transmission electron microscopy (TEM).

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

ISO Certified Reference Material Purity Degree 99% alpha-Arbutin

CAS No.: 84380-01-8

Cosmetics Grade

$77-100/KG FOB

Hederacoside C

CAS No.: 14216-03-6

$1/KG EXW

LICOCHALCONEC

CAS No.: 144506-14-9

$1/KG EXW

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

announcements today(81)

80 20 company(94)

united states logo(227)

single use technology(195)

healthy food name(42)

ups united states(221)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.