Producing PPARgamma2 Knockdown in Mouse Liver
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Last Update: 2020-11-22
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Source: Internet
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Author: User
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A high saturated fat diet induces fatty liver in mice. The fatty liver is triggered initially by an increase in PPARγ2 proteinin the liver, which leads to increased expression of lipogenic genes. Inactivation of PPARγ2 may improve fatty liver inducedby a high saturated fat diet. RNA interference of PPARγ2 should be a powerful tool to elucidate the role of PPARγ2 in thedevelopment of fatty liver. Here, we describe our method for constructing the adenovirus vector containing short hairpin RNAtargeted for PPARγ2. Adenovirus vector-mediated RNA interference for PPARγ2 should be useful for clarifying the biologicalrole of the PPARγ2 pathway in various tissues and for therapeutic application in a variety of diseases.
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