Prof. Zhuoyu Li: Hulu Su E produces chemical sensitivity to colorectal cancer cells by easing the TFAP4/Wnt/β-Catenin signaling path

Original title: Professor Li Zhuoyu: Hulusin E by easing TFAP4/Wnt/β-Catenin signaling pathrax to the colorectal cancer cells chemically increased sensitivity
chemical resistance and toxicity is the limit of 5-fluorouracil (5-fluorouracil, 5-FU) for the treatment of colorectal cancer (colorectal cancer, CRC) treatment of the main obstacles. Therefore, there is an urgent need to identify new advents that make CRC cells sensitive to conventional chemotherapy methods. Cucurbitacin E (CE) is a natural triamcinoid that is widely found in dietary plants and has anti-tumor effects.
this study, Peng Yang, Wen Liu, and Zhuoyu Li, of Shanxi University's Institute of Biotechnology (key laboratory of the Ministry of Chemical Biology and Molecular Engineering), among others, noted that CE enhances the sensitivity of CRC cells to chemotherapy by reducing the expression of box-type transport proteins ABCC1 and MDR1 by weakening adenosine 5'-triphosphate (ATP). Combined with CE's functional magnetite nanoparticles and gene ontogen analysis, it was found that CE binding proteins may involve signaling of the Wnt/β-catenin path. To confirm this finding, β-catenin was raised in drug-resistant cell line, while the synergy between CE and chemotherapy was associated with a reduction in β-catenin expression. In addition, TFAP4 is identified as an in-cell target for CE. It is worth noting that the combination of CE and 5-FU treatment reduced the expression of β-catenin, MDR1 and ABCC1, while TFAP4 over-expression reversed their expression by 2.68±0.46, 0.72±0.44 and 0.93±0.21 times, respectively. Therefore, our results show that CE makes CRC cells sensitive to chemotherapy by reducing signaling of the TFAP4/Wnt/β-catenin pathrapy, indicating that the dietary compound CE can be used as a chemotherapy admissive for CRC therapy.
About The Author of Communication
Recect li Zhuoyu
Li Zhuoyu, female, professor (second level), doctoral tutor, School of Life Sciences, Shanxi University. He is currently dean of the School of Life Sciences of Shanxi University, deputy secretary of the Party Committee, and director of the Institute of Biotechnology of Shanxi University. Professor Li Zhuoyu has been engaged in the research of molecular oncology and natural product drug intervention for many years, mainly focusing on: the regulatory mechanism of key proteins in tumor cell energy metabolism and signaling path, the extraction of functional molecules in natural products and their anti-tumor molecular mechanism, and the molecular toxicity and target screening of environmental factors-induced tumors. Has been in Cancer Research, Oncogene, Oncotarget, Archives of Toxicology, Journal of Agriculture and Food Chemistry and other high-end journals at home and abroad published more than 80 papers, SCI includes more than 60 articles, including high-impact factor papers. Related research has been approved by the national invention patent 8, published in the international gene bank anti-cancer new gene 1. The anti-tumor active substances of rice bran source and local vegetable source were obtained by biotechnology means respectively, which can effectively inhibit tumor growth at cell level and naked mouse level.
this article, "Cucurbitacin E Chemosensitizes Colorectal Cancer Cells via Mitigating TFAP4/Wnt/β-Catenin Signaling", was published online at JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (IF - 4.192).
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