Progress . . . New research results have been obtained in the field of basic food research in Inner Mongolia Autonomous Region.

recently, Professor Wang Junguo of the School of Food Science and Engineering of Inner Mongolia Agricultural University published a study on improving biofilm synthesis based on media buffer salts to improve the freeze-drying survival of Plant Lactobacillus LIP-1 in food chemistry, an internationally renowned academic journal. Rate of rate of Lactobacillus plantarum LIP-1 by by bying biofilm formation on adjusting the composition of the buffer salts in medium, based on the original nuclear chain-specific transcriptional histology to study the effects of different buffer salts on the survival rate of plant Lactobacillus LIP-1 freeze-dried, It was found that the mechanism of increasing the freeze-dried survival of strains was related to the synthesis of biofilms, and the potassium ions in buffer salts could increase the expression of luxS genes in LuxS/AI-2 population sensing systems, while the increase of luxS could promote the expression of cysE genes, which in turn increased the synthesis of biofilms to improve the freeze-dried survival of strains.the study was supported by the National Natural Science Foundation of China Regional Project (high-density fermentation process on the effects of probiotic anti-freezing activity, project number: 31660456). E Jingjing, the first doctoral student to publish a research paper, is the second academic paper to be published in the journal Food Chemistry since the new research results were published in May this year.lactic acid bacteria can improve their resistance to adverse environments by synthesized biofilms. The team found that adding different buffer salts to the medium had a significant effect on the survival rate of plant Lactobacillus LIP-1 freeze-dried, which may be related to biofilm synthesis. Transcriptional data show that potassium ions in buffer salts can increase the expression of LuxS gene in LuxS/AI-2 population sensing system, and increase the synthesis of AI-2 signal molecules. AI-2 signaling molecules are able to increase the expression of the cysE gene, which helps promote the formation of biofilms. By adding biofilm inhibitor D-semi-lactose and conducting real-time fluorescent quantitative polynucleotide chain reaction experiments, the team found that D-semi-lactose inhibited the expression of luxS and cysE genes, reducing biofilm synthesis and freeze-dried survival. The team has shown that using appropriate buffer salts to promote the synthesis of biofilms can improve the freeze-dried survival of strains.paper link:.