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Protein and Vitamin Production in Bacillus megaterium

 Last Update: 2021-01-25
 Source: Internet
 Author: User

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The Gram-positive bacterium
Bacillus megaterium
is frequently employed for the industrial production of exoenzymes including amylases, proteinases, and penicillin acylases, for instance. Here, we describe the utilization of the excellent protein secretion capacity of this bacterium for the production and export of homologous and heterologous recombinant proteins. Necessary genetic tools like various expression vectors, protoplast transformation protocols, and genomic integration strategies are outlined. Practical examples for the directed development of
B. megaterium
to a vitamin B12 production host are also described.

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