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Purification and Characterization of Human Serum and Secretory IgA1 and IgA2 Using Jacalin

 Last Update: 2020-11-24
 Source: Internet
 Author: User

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Human immunoglobulin A (IgA) is found in serum and secretions in several different forms (
1
), Serum IgA is produced by the bone marrow and is pre-dominately monomeric (
M
r= 160 kDa). Most of the IgA in secretions (sIgA) is polymeric, predominantly dimeric, comprising two monomer subunits linkedtogether by intersubunit disulfide bonds and a cysteine-rich polypeptide termed J-chain (
M
r= 16 kDa). Polymeric IgA in secretions, but not in serum, contains an additional heavily glycosylated protein called secretorycomponent (SC) (
M
r= 70 kDa).

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