Purification of serine protease plus no inhibitors?
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Last Update: 2020-11-03
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Source: Internet
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Author: User
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question: I want to purify a serine
protease
, in order to maintain its activity and prevent the degradation of other enzymes, I want to add PMSF, line?
: You can't add PMSF, because PMSF is an irreversible serine
protease inhibitor
that inhibits pyridine Y, protease, Factor Xa, papaya protease, fiber lysase, protease K, herbicide protease, clotting enzyme and trypsin.
Don't know where your serine protease is extracted from, we are also doing the separation and purification of serine protease, generally without inhibitors, but you should operate as low as possible, while the sample should be low temperature or frozen.
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