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Quantification of 13C Enrichments and Isotopomer Abundances for Metabolic Flux Analysis Using 1D NMR Spectroscopy

 Last Update: 2020-11-21
 Source: Internet
 Author: User

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The analysis of stable isotope incorporation following feeding of
13
C-labeled precursors to plant tissues provides the constraints necessary for metabolic flux analysis. This protocol describes the use of one-dimensional
1
H and
13
C nuclear magnetic resonance spectroscopy for the quantification of
13
C enrichments and isotopomer abundances in mixtures of metabolites or hydrolyzed biomass components.

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