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Rapid Detection and Simultaneous Strain Differentiation of Mycobacterium tuberculosis Complex Bacteria by Spoligotyping

 Last Update: 2021-01-26
 Source: Internet
 Author: User

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1 2 butanediol

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The discovery of polymorphic
DNA
in
Mycobacterium tuberculosis
has led to several methods to differentiate climcal isolates. The most widely used method is based on the mobility of the insertion element IS
6110
, which is present in virtually all
M tuberculosis
isolates, usually in multiple copies. IS
6110
-based fingerprinting has been extremely useful for epidemiological studies such as outbreak investigations, assessment of risk factors for recent transmissions, and for studying the dissemination of multiple drug resistant strains (
1
,
2
).

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