Real-time dynamic tracing of the shelling process of a single influenza virus.

Influenza is an acute snort infection caused by influenza A(H1N1), influenza A(H3N2) virus, influenza B Yamagata virus and influenza B Victoria virus.
single virus tracing can reveal the mystery of virus infection in real-time dynamic high space-time and time-to-time resolution.
influenza virus is one of the most threatened viruses in humans. Before
, Zhuang and others had tracked the process by which individual influenza viruses entered their cells.
However, the real-time tracer virus into the cell after the shell ingress process, has been an unachievable scientific problem.
recently, Cui Zongqiang, of the Wuhan Institute of Virusstudies of the Chinese Academy of Sciences, used quantum dot-specific marker virus genome and single-particle tracing technology to dynamically observe the shelling process of a single influenza virus in real time, revealing the dynamic behavior and mechanism of the virus's eight segmentRNA release into the cytoplasm in monomer form and into the nucleus and its dynamic behavior and mechanism within the nucleus.
the team first established a quantum dot-specific marker influenza A influenza Virus genome technology.
in the course of viral infection, the polymerase paso at the top of the viral ribonuclein complex (vRNP) is controlled bioin, binding to the quantum dot specificity of streptomycin-modified, and assembled into mature sub-viral particles to obtain the influenza virus whose genome is labeled by quantum dots.
based on the high brightness of quantum dots and photobleachresistance, the use of single-particle tracing technology, combined with viral envelope and cell component fluorescence markers, the single influenza virus infection of host cells when the shelling process is dynamically visualized in real-time dynamic visual analysis.
real-time dynamics reveal the dynamic process of influenza virus entering late endoscosis 30-90 minutes after infection, membrane fusion from the early endosome to late endoscogenic membrane, separation of genome and viral envelope, release of a single segment gene from the late endosome, release from the release of a single segment gene into the nucleus and the dynamic process of transport in the nucleus.
found that the influenza virus eight vRNPs in the shelling process in a separate rather than group way from the late endothelode sematter, these separate vRNP with characteristic three-stage active transport mechanism into the nucleus, after the nucleation of vRNP in two different diffusion modes transport to its replication / transcription site and other important processes and mechanisms.
using these two techniques, they observed the entire process of the flu virus unshells and the genome entering the nuclei.
this process includes membrane fusion of influenza viruses and host cells, separation of the viral genome from the envelope, RNA of eight segments of the virus released into the cytoplasm in monomer form, a single segment gene enters the nucleus through active transport mechanism, and a single segment gene after nuclearization is transported to replication/retransmission sites in two different diffusion modes.
virus shelling is the process by which a virus RNA is released from the protein shell that wraps it.
The study, the first time in the world, has observed the real-time dynamic process escticness of a single influenza virus shelling and its genome, and is important for a deeper understanding of the life cycle of influenza viruses in host cells, the team leader said.
and will provide ideas for the development of new antiviral pathways.
's findings were published online January 9 in the Proceedings of the National Academy of Sciences (PNAS). Qin Chong, a doctoral student
, is the first author of the thesis and researcher Cui Zongqiang is the author of the communication.
the research was supported by the Chinese Academy of Sciences' strategic leading science and technology project, the national key research and development plan, the National Natural Science Foundation of China, the Chinese Academy of Sciences Youth Innovation Promotion Association, etc.
Source: Xinhua.com, Wuhan Virus Research Institute.