Recombinant Protein Expression in the Baculovirus-Infected Insect Cell System
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Last Update: 2020-12-16
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Source: Internet
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Author: User
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Over the last two decades, the use of eukaryotic cells for expression of recombinant proteins has become the preferred choicefor many applications. This is primarily the case when posttranslational modifications and correct disulfide-bond formationare necessary for protein folding and activity. Among the eukaryotic expression systems, the baculovirus-infected insect cellplatform has gained particular attention, resulting in the development and implementation of multiple strategies for proteinexpression. Here, we present baculovirus-infected insect cells as an efficient expression system for eukaryotic proteins. We demonstrate a simplified and a shortened procedure for recombinant virus production that is sufficient for large-scaleproduction of proteins in insect cells.
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