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Regulation of Murine Interferon Regulatory Factor Gene Expression in the Central Nervous System Determined by Multiprobe RNase Protection Assay

 Last Update: 2021-02-18
 Source: Internet
 Author: User

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Despite a key role in the regulation of interferon (IFN) gene expression and in mediating many downstream actions of the IFNs, the regulation of IFN regulatory factor (
IR
F) gene expression in the normal and pathological central nervous system and, indeed, other tissues, is poorly defined. We sought to examine the expression and regulation of the IRF genes in a number of different murine models for immune- or virally induced central nervous system disease. Here, we describe the development of a multiprobe RNase protection assay that permits the simultaneous detection and quantification of IRF ribonucleic acid transcripts for multiple family members in murine cells and tissues.

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