Reject sodium cyclamate and detect the false "sweet" in liquor by Ion Chromatography

"No wine, no ceremony, no banquet, no wine, no custom" wine culture is a holiday atmosphere assistant for family reunion, visiting relatives and friends Between the glasses of wine, swaying dreams, sending blessings to each other, sweet and warm Unexpectedly, sweet and happy festivals are also full of unsweetened embarrassment - a well-known liquor dealer reported the addition of sodium cyclamate in his own liquor, and the event continued to ferment, causing widespread concern A stone stirs up a thousand waves, so the problem comes! What is the sweet element? Sodium cyclamate, also known as sweetener and chemical name - sodium cyclamate, is a kind of white crystalline powder sweetener, whose sweetness is 30-40 times that of sucrose, which is commonly used in food production Tips: sweetener, synthetic, 30-40 times sweetness of sucrose Is it harmful to human body? In 1969, the Research Committee of the National Academy of Sciences received animal experimental evidence that the mixture of cyclamate and saccharin can cause bladder cancer In 1970, the U.S Food and Drug Administration issued a comprehensive ban on the use of sodium cyclamate Countries such as Britain, Japan and Canada subsequently banned it Can we add sodium cyclamate to liquor? The hygienic standard for the use of food additives (GB 2760-2014) strictly limits the amount of sodium cyclamate used in food processing Among them, it is forbidden to add sodium cyclamate in liquor How to detect the sodium cyclamate forbidden to be added in liquor? The national food safety standard GB 5009.97-2016 determination of sodium cyclamate in food stipulates three determination methods of sodium cyclamate in food - gas chromatography, liquid chromatography and liquid chromatography-mass spectrometry / mass spectrometry The reaction of cyclohexanol and similar substances of cyclohexyl group in liquor may react with sodium nitrite, which is mistaken for the reaction of sodium cyclohexylaminosulfonate and sodium nitrite, which may result in the false-positive determination of sodium cyclamate in liquor When the operation is complex and the matrix of the sample is complex, the same problem may be encountered in the derivation of gas chromatography Liquid chromatography-mass spectrometry / mass spectrometry liquid chromatography-mass spectrometry / mass spectrometry is suitable for the determination of cyclamate in liquor The pretreatment requires water bath evaporation to remove the ethanol matrix, and the cost of liquid quality detection is slightly higher The cost of "tipsy" is higher and higher Ion chromatography (IC) is simple, fast, economical and environmentally friendly Gamma Dionex Gamma Aquion Gamma RFIC ion chromatograph ion chromatography (IC) - ion exchange principle, excellent separation, qualitative and quantitative chromatography of polar ionic compounds "Water only" ion chromatography (RFIC) - electrolytic water produces eluent and inhibitor The operation of the instrument requires only ultrapure water, which is a very simple instrument analysis scheme Principle diagram of "water only" ion chromatograph principle diagram of eluent generator (eg) principle diagram of electrolytic suppressor principle diagram of sodium cyclamate, strong water solubility, easy to ionize, exist in the form of sulfonate anion under alkaline conditions, ion exchange separation and detection is the best analytical hand of Zui, without any derivative operation For liquor samples, the sample can be directly injected for analysis after simple dilution The detection limit of sodium cyclamate in liquor by ion chromatography is 0.072mg/l, which is equivalent to the liquid chromatography-mass spectrometry / mass spectrometry in GB 5009.97-2016 determination of sodium cyclamate in food In addition, through the optimization of chromatographic conditions, ion chromatography, one-time injection can also simultaneously determine artificial sweeteners such as Acesulfame and saccharin sodium, as well as common inorganic anions in water quality such as chloride ion, nitrate and sulfate which have an impact on the taste of liquor (figure below) Is it more than one stroke! The results of ion chromatography of various sweeteners (sodium cyclamate, acesulfame and saccharin) were determined at the same time by ion chromatography What should we do if we want to verify it by tandem mass spectrometry? Semefei electrolytic suppressor, when inhibiting conductivity detection, has transformed strong alkaline anion eluant (such as potassium hydroxide) into water In other words, if ion chromatography wants to be connected in series with mass spectrometry, it can be directly connected Scan the QR code below to get the whole industry solution of Symantec, or pay attention to the public Hao of "Symantec chromatography and mass spectrometry China" for more information+