Repair and maintenance of microbial fermentation equipment

Microbial fermentation channel: In order to ensure the normal operation of the fermentation tank, the user must be familiar with the working principle of the equipment and the structure of the components, performance and the role of pipelines, valves, should also pay attention to and strengthen the maintenance and maintenance of equipment, only to ensure that the equipment in the best state, extend the working life of the equipment"text-align: center;"img src""alt"""microbial fermentation equipment repair and maintenance" title"daily inspection and maintenance of fermentation tank systemthe maintenance and maintenance of fermentation tank system can be divided into two ways, bothdaily inspection and maintenance (the monitoring contents of the equipment in operation should be recorded)system pressure, tank pressure is stable in the specified rangeWhether the mixing system, the temperature control system and the solenoid valve sound correctWhether the culture liquid color is correctWhether the temperature and dissolved oxygen PH display parameters are in accordance with the settingsWhether the fluid level in the tank is correctValve fittings, each interface is normal, there is no leakageregular inspection and maintenanceare required for three months, and the equipment needs to be overhauled and correctedCut off all power, water, gas (vapor) sourcesCheck carefully whether all seals and seals of the system are correctIf there is permanent deformation, aging, scratches, damage, must be replacedCheck the integrity of the sterilizing filter, there is damage, blockage phenomenon to be replaced Motor carbon brush wear and rectifiers, bearings, joints, mixing system conditions Check all switches, buttons, electrical appliances, electronic components, fixing screws, nuts for loose, heating phenomenon the cleaning and maintenance of the fermentation tank cleaning the container is the place of direct contact with the material The degree of surface finish and the cleanliness of the internal components are the factors that directly affect the contamination of bacteria Therefore, after each fermentation and before re-use must be timely cleaning tank and related equipment, cleaning should pay attention to electrical components, electric ity interface, can not enter water, moisture Before cleaning should remove PH, DO electrode according to its requirements for maintenance (see electrode maintenance) cleaning tank can be combined with intake air, motor stirring, heating together, such as multiple water changes can not be cleaned, to open the top cover with soft brush washing tank internal parts The method is as follows: control switch and power switch, remove the top cover electrode, motor and its connected plug Remove the intake hose condenser quick connector remove the hose and connector on the intake filter, remove the top cover vertically and upward along with stirring, and place it horizontally on a flat table top, pad, do not collide the shaft and impeller, and brush the tank and internal components with neutral detergent note: the tank cover can only grasp the bushing or top cover when moving and cleaning, can not grasp the mixing shaft, impeller and other easily deformed parts At the same time, the cleaning should be its tank and export valve and other O-shaped seals to check, such as the discovery of permanent deformation, aging, scratches and other phenomena need to be replaced in a timely manner After cleaning and reinstalling, dry with a rag for its exterior and tank racks Tighten the tank cover with six fastening screws, balance vigorously and pay attention to the balance between the tank and the tank holder For the cleaning of glass fermentation tank should be particularly careful, do not collide when disassembling, tighten the lid tightening screws, force must be balanced, and pay attention to the balance of the gap, has been free of damage to the glass tank sealing test the electrode, motor, cable, in coming in and out of the gas hose, condenser in and out of the water connector installed in place; Methods are as follows: close the 401, 304, 106 valves, tighten each interface, plug, electrode fastening cap on the tank, open 207, 208, 209 valves and air compressor, adjust the 206 valve to keep the tank pressure around 0.2MPa, pull off the fast connector on the DX, the tank pressure drop speed should be less than 0.02Mpa/h The system for 2-3 hours of trial operation, if there are problems to deal with the corresponding, can be formally used fermentation tank to be used if it needs to be cultured and fermented again in the short term, it should be sterilized Then into the sterile air, hold the pressure to be used If ready for long-term deactivation, it should be sterilized, and then put out the water tank and tank memory water, relax the tank cover tightening screws, remove the electrode maintenance and storage, the tank, the pipe residual water released clean, close all valves, power supply, cover the dust cover After secondary cultivation, use a clean cloth to remove electrical boxes, cables and other connectors and other tank components precautions the output pressure of the steam generator should be checked and adjusted before sterilization to ensure that the pressure is 0.35Mpa (the steam generator pressure gauge display value) During sterilization, the air pressure in the tank should be checked to ensure that the pressure is .lt;0.15Mpa the output pressure of the air compressor should be checked and adjusted before fermentation, to ensure that the pressure is 0.4Mpa (the air compressor pressure gauge display value), and the pressure relief valve output pressure should be 0.25Mpa During fermentation, the air pressure in the tank should be checked to ensure that the pressure is 0.10Mpa Do, pH electrode use and maintenance must be as required, otherwise it is very vulnerable to damage All electrical devices are strictly prohibited from entering water, moisture or pollution Any questions, please contact us as follows The maintenance mixing system of the mixing system includes the mixing shaft, the mixing slurry, and the mechanical seal Bearings, shafters and motors, etc daily maintenance: check the operation of the mixing shaft, the heating of the motor, and the emission of abnormal noise, and check the bolts for looseness annual inspection of the wear of the mechanical seal to ensure the normal operation of the mechanical seal, to avoid unnecessary contamination may be if the use of double-ended mechanical sealing, to always pay attention to the change of sterile water surface, if the liquid surface drop, indicating that the seal gap leakage, should be treated immediately after fermentation annual inspection of the oil seal and motor carbon brush wear, as well as oil seals and bearingwear the maintenance and maintenance of the sterile air system the sterilized air system includes air compressors, coarse filters, fine filters, diaphragm valves and air distributors In the daily maintenance, we should pay attention to the operation of the air compressor, in the fermentation process should be the gas tank discharge valve drain twice a day Filter maintenance and maintenance sterilization temperature should be controlled below 125 degrees C in the sterilization process should pay attention to the filter sterilization temperature should be controlled below 125 degrees C Because the filter material is too high at temperature, its binder will produce a shedding and cause the filter to fail filter filter filter replacement because the air contains a lot of dust, the filter after a period of use, the filter filter micropores will gradually block, so that on the one hand lead to increased resistance, resulting in a serious shortage of air flow and air pressure drop increase, thus affecting the amount of ventilation, and may cause bacteria Replacement situ at this time because of the large differences in air quality, so no accurate service life, generally up to 6 months or more The replacement standard is 1.5 times the minimum air flow and not less than the tank volume (The pressure difference between the relief device outlet and the tank pressure is not less than 1kg/cm2) each fermentation ends, the air distributor needs to be cleaned If the fermentation solution is similar in nature to water, water can be added to the tank and then cleaned with compressed air If the fermentation solution is more viscous, or contains fine particles, it is best to remove the air distributor, then unscrew the plug and rinse with water At the same time to ensure that the small hole ventilation smooth The maintenance and maintenance of the steam system the steam system includes steam filters, steam line valves, etc Always take care to ensure a constant vapor pressure during use the filter element of the steam filter will also be blocked after use and the filter element needs to be replaced In the course of operation, should pay attention to open the steam valve, do not immediately open, this will impact the steam filter filter, in severe cases will break the filter or leak to make it fail the maintenance of the dissolved oxygen electrode if you do not need to remove the electrode from the tank for a long time, put it in the electrode box If the sensitive film is dirty, rinse it clean with clean water, and if it is not rinsed off, do not scrape with any hard objects to avoid hanging sensitive sensitive film Do not use liquids such as water to soak or vapor to erode cable and electrode connectors Do not scrape sensitive film with a sharp etre to avoid damage how to change membrane soldering and electrolyte METTLER TOLEDO oxygen electrode factory after a series of tests, the electrode itself with an electrode membrane, but because in the purchase and transportation process requires a certain period of time, so it is best to replace the electrolyte before use If the electrode signal produces errors (long response time, mechanical damage, increased current in anoxygenic medium, etc.), the membrane needs to be replaced, the maintenance of the electrolyte is replaced, every three months: place the electrode in a vertical position and unscrew the old electrode film Rinse the inner electrode body with water and dry carefully with paper, check for electrolytes between the inner electrode body and the stainless steel housing, if there is residual electrolyte, and gently shake it clean After a period of use, if found that the electrode body silver ring blackened, can be used to more than 1000 pieces of fine sandpaper polish can be Check the O-ring and spring for mechanical damage and replace it if necessary Pour the electrolyte into the membrane to be replaced, the liquid surface control below the thread, appropriate additional electrolyte, to avoid less electrolyte and affect electrode polarization, the electrode is placed in a vertical position and the membrane gently screwed on the electrode body, screw the electrode film pay attention to the "into two back one" principle, to avoid the film can not be discharged in time due to the film, the final electrode film should be tightened, no O seal color (black) to seep out of the electrolytic Due to the sealing of the O-ring, the membrane will be easily tightened, if you need to tighten hard or this process can not be easily completed, may not be screwed in the correct position After each membrane change or electrolyte, the electrode must be re-polarized and polarized (see operating instructions): after replacing a new electrolyte or changing a new membrane, it must be powered on continuously for more than 7 hours, i.e polarized, so that accurate calibration can be performed Polarize first, then calibrate calibration: the specific procedure is detailed in the oxygen transmitter operating instructions the maintenance of the solenoid valve solenoid valve is used for the cooling of the fermentation tank, the solenoid valve core is stuck, the valve core seal failure and the damage of the solenoid coil can cause the failure of the solenoid valve fault judgment: cooling valve failure: open solenoid valve, solenoid valve upper end face no magnetism, no cooling water discharge, coil bad; In the case of ensuring the operation of the circulating pump and the smooth flow of the circulating line, the biggest reason for the temperature drop does not rise is the solenoid valve leakage or valve core stuck, and the other reason may be that the electric heater burned out other maintenance the maintenance of the trap valve: since the seal inside the ball valve is made of two more hemisco-like tetrafluoroethylene, it may leak between the seal and the valve core after long use Service method: the card sleeve screw at both ends of the loosen valve, and then tighten the cylindrical joint at both ends of the valve, you can (Normally: turn the valve handle should have a certain amount of resistance) card sleeve connector leakage: tightening the pressure cap on the joint can be The maintenance of the discharge valve: turn the valve cover open, pull out the valve core, check that the seal ring is intact, otherwise replace the seal ring also need to pay attention to some problems: 1, the environment of placement equipment should be clean, dry, well ventilated, water, steam should not be directly poured on the appliance 2, after the device is activated, must be cleaned in time to prevent fermentation fluid drying in, 3, all kinds of instruments, should be required in accordance with the requirements of maintenance and storage pressure gauges, safety valves; 4, equipment should be cleaned when the equipment is discontinued, blow-drying; 5, air compressor around the environment should be kept clean, fresh air, drainage Groove; 6, air storage tank regularly open the tank bottom of the sewage valve, drain the cold inside the tank; 7, cold dryer should be regularly pressed to open the key (green button) discharge inside the cold; 8, after the equipment is activated, must be cleaned in time to prevent the fermentation liquid dry knot in the fermentation tank and lines, valves 9, all kinds of instruments, should be in accordance with the requirements of maintenance and storage pressure gauges, safety valves, temperature meters should be calibrated once a year 10, equipment should be cleaned and dried when the equipment is discontinued Filter filter should be removed cleaning, cool dry, proper lying, flange pressing nut should be loosened to prevent permanent deformation of the seal ring 11, the environment around the air compressor should be kept clean, fresh air, drains for living water, the surface of the body clean 12, the air storage tank regularly open the tank bottom of the sewage valve, drain the condensate in the tank (about 30 days or so to deal with once, the specific situation should be more local air humidity) 13, cold dryer should be regularly pressed to open the key (green button) discharge inside the condensate : maintenance, cleaning and maintenance of the fermentation tank system