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In this chapter, we examine important parameters that affect the performance of replaceable polymers currently used for
DNA
sequencing by capillary electrophoresis (DNASCE). Background on physical models used to describe polymer solutions and DNA migration in semi-dilute polymer solution will be given to provide a framework for interpretation of experimental data and provide estimates of optimized polymer and electrophoresis parameters. Discussion of parameters affecting DNASCE resolution will be followed by comparison of experimental conditions for four linear polymers currently used in DNASCE (
1
–
10
). Recent developments in performance optimization and inner-wall coatings will also be discussed.