Research on the efficiency of photo-cooperation.

The well-known phenomenon of "everything grows by the sun" is based on the occurrence of oxygenated photochemicals in organisms such as higher plants, algae and blue bacteria.
these organisms use photochemical fixation of CO2 to convert solar energy into chemical energy for storage, while cracking water molecules and releasing oxygen for biospiration.
is the most important biochemical reaction on Earth, providing the material basis on which life on Earth depends.
, improving the efficiency of photocodescing is of great significance to the effective use of solar energy, the promotion of agricultural production and income, the acceleration of industrial CO2 emission reduction and resource utilization.
photoynsy is an extremely complex bio-chemical process.
depending on whether light is needed, photodynsms are man-madely divided into light and dark reactions.
Previous studies on retrofitted photocodesi have focused on how to improve the efficiency of photoreactive energy utilization and transformation, or the carbon sequestration efficiency of Rubisco, the key enzyme for dark reactions, with little consideration for improving the coupled efficiency of light reactions and dark reactions.
in the actual physiological process, photocrysmisive light and dark reactions are inextricable organic whole.
light reaction produces energy (ATP) and reduction force (NADPH), while dark reactions consume ATP and NADPH to achieve a fixed reduction of CO2.
The Li Wei Research Group of the Institute of Microbiology of the Chinese Academy of Sciences, in view of the basic problem that ATP produced by photoreactive energy cannot meet the energy demand of dark reaction carbon sequestration, according to the basic principle of the joint production of ATP and NADPH in light reaction, the photoreaction and dark reaction of photomodulation as an organic whole are viewed from the global perspective of cells. NADPH, which connects photorescing and dark reactions, presents a new idea to import NADPH consumption module, thus breaking the inherent NADPH balance of cells, and enhancing the internal driving force of photoreactive reaction and improving the efficiency of photocodying through the effective coupling of photoreactive and dark reaction.
using photochemical oxygenated blue bacteria as a model, the researchers created a biosynthetic pathway that consumed only NADPH without additional consumption of ATP by introducing NADPH-dependent dehydrogenase (see figure).
series of photosynthic physiological and bio-chemical analyses showed that after the introduction of NADPH consumption pathway, cell growth was significantly accelerated, photogenic efficiency was increased by about 50%, and cell activity was higher.
also found that the light saturation point of the modified blue bacteria doubled, indicating that it can withstand higher light strength, which is important for adapting to the dramatic changes in light strength in nature.
The results show that reductive force-driven cell global metabolic engineering strategy can improve photochrescency efficiency more effectively than traditional single modification light reaction or dark reaction, which is also of reference value for the modification of photochemical organisms.
results were published online August 4 in the journal Metabolic Engineering.
(the study was funded by the National Natural Science Foundation of China and the Chinese Academy of Sciences for the key deployment of the project "artificial biological transformation of carbon dioxide."
, an associate researcher, and Zhang Fuliang, a doctoral student, are co-authors of the paper.
source: China Biotechnology.