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Restriction Fragment Length Polymorphism Typing of Mycobacteria

 Last Update: 2021-02-13
 Source: Internet
 Author: User

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In principle, restriction fragment length polymorphism (RFLP) typing can be applied to strains of all mycobacterial species for which suitable probes have been identified. International consensus has been achieved regarding the methodology of IS
6110
RFLP typing of
Mycobacterium tuberculosis
complex isolates (
1
) and IS
1245
RFLP typing of
Mycobacterium avium
strains (
2
). This chapter describes the technical details of these standardized methods regarding the isolation of
DNA
, restriction enzymes, electrophoresis conditions, internal- and external-size markers, Southern blotting, and several probes used for hybridization. Furthermore, RFLP typing of isolates of some other mycobacterial species is described.

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